the bubble like shapes are yeast, and the little lines are bacteria.
by Sweaty-Importance972
1 Comment
VictorQuartermain
Hey, cool picture, but I’d like to clarify. The bubble like structures are starch granules. Yeast cells are aproximately 10 micrometers, wich you need an amplification of 1000x to truly observe. Use immersion oil to let the objective slide onto the glass. Now this is really cool. Use methylene blue to stain the cells. Living yeast cells will reduce the methylene blue and turn transparant, except for their membranes. Dead yeast cells are unable to reduce the methylene blue, since they’re dead. They will turn fully blue.
If you want to see bacteria you need to do a gram-stain. Bacteria are approximatley 10 times smaller than yeast cells.
1 Comment
Hey, cool picture, but I’d like to clarify. The bubble like structures are starch granules. Yeast cells are aproximately 10 micrometers, wich you need an amplification of 1000x to truly observe. Use immersion oil to let the objective slide onto the glass. Now this is really cool. Use methylene blue to stain the cells. Living yeast cells will reduce the methylene blue and turn transparant, except for their membranes. Dead yeast cells are unable to reduce the methylene blue, since they’re dead. They will turn fully blue.
If you want to see bacteria you need to do a gram-stain. Bacteria are approximatley 10 times smaller than yeast cells.