Schwingshackl, L., Heseker, H., Kiesswetter, E. & Koletzko, B. Dietary fat and fatty foods in the prevention of non-communicable diseases: A review of the evidence. Trends Food Sci. Technol. 128, 173–184 (2022).
Google Scholar
Nettleton, J., Brouwer, A., Geleijnse, J. & Hornstra, G. Saturated fat consumption and risk of coronary heart disease and ischemic stroke: a science update. Ann. Nutr. Metab. 70, 26–33 (2017).
Google Scholar
Cao, X. et al. The effect of MUFA-Rich food on lipid profile: A meta-analysis of randomized and controlled-feeding trials. Foods 11, 1982. https://doi.org/10.3390/foods1113198 (2022).
Google Scholar
Law, M. Dietary fat and adult diseases and the implications for childhood nutrition: an epidemiologic approach. Am. J. Clin. Nutr. 72 (Suppl), 1291S–1296S (2000).
Google Scholar
Nestel, P. & Mori, T. Dairy foods: is its cardiovascular risk profile changing? Curr. Atheroscler Rep. 24, 33–40 (2022).
Google Scholar
Allman-Farinelli, M. et al. A diet rich in high-oleic-acid sunflower oil favorably alters low-density lipoprotein cholesterol, triglycerides, and factor VII coagulant activity. J. Am. Diet. Assoc. 105, 1071–1079 (2005).
Google Scholar
Temme, E., Mensink, R. & Hornstra, G. Comparison of the effects of diets enriched in lauric, palmitic or oleic acids on plasma lipids and lipoproteins in healthy women and men. Am. J. Clin. Nutr. 63, 897–903 (1996).
Google Scholar
Kromhout, D. et al. Comparative ecologic relationships of saturated fat, sucrose, food groups, and a mediterranean food pattern score to 50-year coronary heart disease mortality rates among 16 cohorts of the seven countries study. Eur. J. Clin. Nutr. 72, 1103–1110 (2018).
Google Scholar
Kumar, M., Sambaiah, K. & Lokesh, B. Hypocholesterolemic effect of anhydrous milk fat ghee is mediated by increasing the secretion of biliary lipids. J. Nutr. Biochem. 11, 69–75 (2000).
Google Scholar
Zommara, M. Hypocholesterolemic effect of milk fat and Olive oil in C57BL/6 N mice fed an atherogenic diet. J. Agric. Res. Mansoura Univ. 27, 3995–4004 (2002).
Sharma, H., Zhang, X. & Dwivedi, C. The effect of ghee (clarified butter) on plasma lipid levels and microsomal lipid peroxidation. AYU. 31, 134–140 (2010).
Google Scholar
Fontecha, J. et al. Milk and dairy product consumption and cardiovascular diseases: an overview of systematic reviews and meta-analyses. Adv. Nutr. 10 (Suppl 2), S164–S189 (2019).
Google Scholar
Gisterå, A., Ketelhuth, D., Malin, S. & Hansson, G. Animal models of atherosclerosis-supportive notes and tricks of the trade. Circ. Res. 130, 1869–1887 (2022).
Google Scholar
Perdomo, L. et al. Protective role of oleic acid against cardiovascular insulin resistance and in the early and late cellular atherosclerotic process. Cardiovasc. Diabetol. 14, 75. https://doi.org/10.1186/s12933-015-0237-9 (2015).
Google Scholar
Lu, Y. et al. Protective effects of oleic acid and polyphenols in extra virgin olive oil on cardiovascular diseases. Food Sci. Hum. Well. 13, 529–540 (2024).
Google Scholar
Harvatine, K., Dale, E. & Mark, A. Mammary gland, milk biosynthesis and secretion: milk fat in Encyclopedia of dairy sciences (ed. McSweeney, P., McNamara, J.) 190–197. (Academic Press, 2022).
Rodríguez-Alcalá, L. et al. Milk fat components with potential anticancer activity-a review. Biosci. Rep. 37, BSR20170705. https://doi.org/10.1042/BSR20170705 (2017).
Google Scholar
Abdelhalim, K. Short-chain fatty acids (SCFAs) from gastrointestinal disorders, metabolism, epigenetics, central nervous system to cancer – A mini-review. Chem. Biol. Interact. 388, 110851. https://doi.org/10.1016/j.cbi.2023.110851 (2024).
Google Scholar
Mathiasen, S. et al. Novel methodology to enrich medium- and short-chain fatty acids in milk fat to improve metabolic health. Food Funct. 15, 7951–7960 (2024).
Nicolosi, R. et al. Decreased aortic early atherosclerosis in hypercholesterolemic hamsters fed oleic acid-rich TriSun oil compared to linoleic acid-rich sunflower oil. J. Nutr. Biochem. 13, 392–402 (2002).
Google Scholar
Starčević, K. et al. Growth performance, plasma lipids and fatty acid profile of different tissues in chicken broilers fed a diet supplemented with linseed oil during a prolonged fattening period. Veterinarski Arhiv. 84, 75–84 (2014).
Starčević, K. et al. Production performance, meat composition and oxidative susceptibility in broiler chicken fed with different phenolic compounds. J. Sci. Food Agric. 95, 1172–1178 (2015).
Google Scholar
Peña-Saldarriaga, L., Pérez-Alvarez, J. & Fernández-López, J. Quality properties of chicken emulsion-type sausages formulated with chicken fatty byproducts. Foods 9, 507. https://doi.org/10.3390/foods9040507 (2020).
Google Scholar
Lin, L. & &Tan, F. Influence of rendering methods on yield and quality of chicken fat recovered from broiler skin. Asian-Australaian J. Anim. Sci. 30, 872–877 (2017).
Google Scholar
Kochhar, P. Thermal stability of fats for high temperature applications in Functional dietary lipids (ed. Sanders, T.) 103–148. https://doi.org/10.1016/B978-1-78242-247-1.00005-3 (Woodhead Publishing, 2016).
Liu, M., Wang, D., Black, D. & Tso, P. Differential effect of four-week feeding of different dietary fats on the accumulation of fat and the cholesterol and triglyceride contents in the different fat depots. Nutrients 12, 123241. https://doi.org/10.3390/nu12113241 (2020).
Google Scholar
Limmatvapirat, C. et al. Beef tallow: extraction, physicochemical property, fatty acid composition, antioxidant activity, and formulation of lotion bars. J. Appl. Pharm. Sci. 11, 18–28 (2021).
Google Scholar
Nogoy, K. et al. Fatty acid composition of grain- and grass-fed beef and their nutritional value and health implication. Food Sci. Anim. Resour. 42, 18–33 (2022).
Google Scholar
Ruuth, M. et al. Overfeeding saturated fat increases LDL (low-density lipoprotein) aggregation susceptibility while overfeeding unsaturated fat decreases proteoglycan-binding of lipoproteins. Arterioscler. Thromb. Vasc. Biol. 41, 2823–2836 (2021).
Google Scholar
Gouvinhas, I. et al. Critical review on the significance of olive phytochemicals in plant physiology and human health. Molecules 22, 1986. https://doi.org/10.3390/molecules22111986 (2017).
Google Scholar
Bucciantini, M. et al. Olive polyphenols: antioxidant and anti-inflammatory properties. Antioxidants 10, 1044. https://doi.org/10.3390/antiox10071044 (2021).
Google Scholar
Nedkoff, L. et al. Global trends in atherosclerotic cardiovascular disease. Clin. Ther. 45, 1087–1091 (2023).
Google Scholar
Milena, E. & Maurizio, M. Exploring the cardiovascular benefits of extra virgin olive oil: insights into mechanisms and therapeutic potential. Biomolecules 15, 284. https://doi.org/10.3390/biom15020284 (2025).
Google Scholar
Xia, M., Zhong, Y., Peng, Y. & Qian, C. Olive oil consumption and risk of cardiovascular disease and all-cause mortality: A meta-analysis of prospective cohort studies. Front. Nutr. 18, 1041203. https://doi.org/10.3389/fnut.2022.1041203 (2022).
Schwingshackl, L. et al. Olive oil in the prevention and management of type 2 diabetes mellitus: a systematic review and meta-analysis of cohort studies and intervention trials. Nutr. Diabetes. 10, e262. https://doi.org/10.1038/nutd.2017.12 (2017).
Google Scholar
Mazzocchi, A., Leone, L., Agostoni, C. & Pali-Schöll, I. The secrets of the Mediterranean diet. does [only] olive oil matter?. Nutrients 11, 2941. https://doi.org/10.3390/nu11122941 (2019).
Google Scholar
Borghjid, S. & Feinman, R. D. Response of C57Bl/6 mice to a carbohydrate-free diet. Nutr. Metab. 9, 69. https://doi.org/10.1186/1743-7075-9-69 (2012).
Google Scholar
Song, H. K. & Hwang, D. Y. Use of C57BL/6 N mice on the variety of immunological researches. Lab. Anim. Res. 33, 119–123. https://doi.org/10.5625/lar.2017.33.2.119 (2017).
Google Scholar
Thiex, N., Anderson, S. & Gildemeister, B. Crude fat, hexanes extraction, in feed, cereal grain, and forage (Randall/Soxtec/submersion method): collaborative study. J. AOAC Int. 86, 899–908 (2003).
Google Scholar
Abou-Donia, S. Origin, history and manufacturing process of Egyptian dairy products: an overview. Alex. J. Food Sci. Technol. 5, 51–62 (2008).
Reeves, P., Nielsen, F. & Fahey, C. AIN-93G purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939–1951 (1993).
Google Scholar
Ishinaga, M., Sugiyama, S. & Mochizuki, T. Daily intakes of fatty acids, sterols, and phospholipids by Japanese women and serum cholesterol. J. Nutr. Sci. Vitaminol. 40, 557–567 (1994).
Google Scholar
Ikeda, I. et al. α-Linolenic, eicosapentaenoic and docosahexaenoic acids affect lipid metabo-lism differently in rats. J. Nutr. 124, 1898–1906 (1994).
Google Scholar
Du, C. et al. Cholesterol synthesis in mice suppressed but Lipofuscin formation is not affected by long-term feeding of n-3fatty acid-enriched oils compared with lard and n-6 fatty acid-enriched oils. Biol. Pharm. Bull. 26, 766–770 (2003).
Google Scholar
Bligh, E. & Dyer, W. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917 (1959).
Google Scholar
Sperry, W. M. & Weeb, M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J. Biol. Chem. 187, 97–106 (1950).
Google Scholar
Fletcher, M. A colorimetric method for Estimation of plasma triglycerides. Clin. Chim. Acta. 22, 393–397 (1968).
Google Scholar
Bartlett, G. Phosphorus assay in column chromatography. J. Biol. Chem. 234, 466–468 (1959).
Google Scholar
Kannan, S. et al. LDL-cholesterol: Friedewald calculated versus direct measurement-study from a large Indian laboratory database. Indian J. Endocrinol. Metab. 18, 502–504 (2014).
Google Scholar
Dobiasova, M. AIP-Atherogenic index of plasma as a significant predictor of cardiovascular risk: from research to practice. Vnitr. Lek. 52, 64–71 (2006).
Google Scholar
Yin, B. et al. Non-linear association of atherogenic index of plasma with insulin resistance and type 2 diabetes: a cross-sectional study. Cardiovasc. Diabetol. 22, 157. https://doi.org/10.1186/s12933-023-01886-5 (2023).
Google Scholar
Bittner, V. et al. The triglyceride/high-density lipoprotein cholesterol ratio predicts all-cause mortality in women with suspected myocardial ischemia: a report from the women’s ischemia syndrome evaluation (WISE). Am. Heart J. 157, 548–555 (2009).
Google Scholar
Sun, T. et al. Predictive value of LDL/HDL ratio in coronary atherosclerotic heart disease. BMC Cardiovasc. Disord. 22, 273. https://doi.org/10.1186/s12872-022-02706-6 (2022).
Google Scholar
Calling, S. et al. The ratio of total cholesterol to high density lipoprotein cholesterol and myocardial infarction in women’s health in the Lund area (WHILA): a 17-year follow-up cohort study. BMC Cardiovasc. Disord. 19, 239. https://doi.org/10.1186/s12872-019-1228-7 (2019).
Google Scholar
Zhou, D. et al. The effect of total cholesterol/high-density lipoprotein cholesterol ratio on mortality risk in the general population. Front. Endocrinol. 15, 1012383. https://doi.org/10.3389/fendo.2022.1012383 (2022).
Paigen, B. et al. Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis 68, 231–240 (1987).
Google Scholar
Ni, W., Tsuda, Y., Sakono, M. & Imaizumi, K. Dietary soy protein isolate, compared with casein, reduces atherosclerosis lesion area in Apolipoprotein E-deficient mice. J. Nutr. 128, 1884–1889 (1998).
Google Scholar
SPSS. SPSS for windows. Statistical package for social studies 567 Software; Version 24; (Ibm Corp., 2016).
Hoffmann, H. M. Determination of reproductive competence by confirming pubertal onset and performing a fertility assay in mice and rats. J. Vis. Exp. 13, 58352. https://doi.org/10.3791/58352 (2018).
Google Scholar
Nizar, N., Marikkar, J. & Hashim, D. Differentiation of lard, chicken fat, beef fat and mutton fat by GCMS and EA-IRMS techniques. J. Oleo Sci. 62, 459–464 (2013).
Google Scholar
FAO. Fats and fatty acids in human nutrition: report of an expert consultation. FAO Food Nutr. 91, 1–166 (2010).
Dehghan, M. et al. Association of dairy intake with cardiovascular disease and mortality in 21 countries from five continents (PURE): a prospective cohort study. Lancet 392, 2288–2297 (2018).
Google Scholar
Dinh, T. & To, K. Wes schilling, M. Fatty acid composition of meat animals as flavor precursors. Meat Muscle Biol. 34, 1–16. https://doi.org/10.22175/mmb.12251 (2021).
Google Scholar
Podrini, C. et al. High-fat feeding rapidly induces obesity and lipid derangements in C57BL/6 N mice. Mamm. Genome. 24, 240–251 (2013).
Google Scholar
Kopp, W. How western diet and lifestyle drive the pandemic of obesity and civilization diseases. Diabetes Metab. Syndr. Obes. 12, 2221–2236 (2019).
Google Scholar
Wondmkun, Y. Obesity, insulin resistance, and type 2 diabetes: associations and therapeutic implications. Diabetes Metab. Syndr. Obes. 13, 3611–3616 (2020).
Google Scholar
Hwang, K., Tung, H., Lu, Y. & Shaw, H. Liquid chicken oil could be a healthy dietary oil. J. Oleo Sci. 70, 1157–1164 (2021).
Google Scholar
Aloysius, T. et al. Plasma cholesterol- and body fat-lowering effects of chicken protein hydrolysate and oil in high-fat fed male Wistar rats. Nutrients 14, 5364. https://doi.org/10.3390/nu14245364 (2022).
Google Scholar
Svenson, K. et al. Multiple trait measurements in 43 inbred mouse strains capture the phenotypic diversity characteristic of human populations. J. Appl. Physiol. 102, 2369–2378 (2007).
Google Scholar
Gordon, S. et al. A comparison of the mouse and human lipoproteome: suitability of the mouse model for studies of human lipoproteins. J. Proteome Res. 14, 2686–2695 (2015).
Google Scholar
Dobiasova, M. et al. Cholesterol esterification and atherogenic index of plasma correlate with lipoprotein size and findings on coronary angiography. J. Lipid Res. 52, 566–571 (2011).
Google Scholar
Ochiai, M. Evaluating the appropriate oral lipid tolerance test model for investigating plasma triglyceride elevation in mice. PLoS One 6, e0235875. https://doi.org/10.1371/journal.pone.0235875 (2020).
Google Scholar
Fang, J. et al. AMPKα pathway involved in hepatic triglyceride metabolism disorder in diet-induced obesity mice following Escherichia coli infection. Aging (Albany NY) 6, 3161–3172. https://doi.org/10.18632/aging.101623 (2018).
Jiang, T. et al. Diet-induced obesity in C57BL/6J mice causes increased renal lipid accumulation and glomerulosclerosis via a sterol regulatory element-binding protein-1c-dependent pathway. J. Biol. Chem. 280, 32317–32325. https://doi.org/10.1074/jbc.M500801200 (2005).
Google Scholar
Kalaany, N. Y. et al. LXRs regulate the balance between fat storage and oxidation. Cell Metabol. 1, 231–244. https://doi.org/10.1016/j.cmet.2005.03.001 (2005).
Google Scholar
Montgomery, M. K. et al. Association of muscle lipidomic profile with high-fat diet-induced insulin resistance across five mouse strains. Sci. Rep. 7, 13914. https://doi.org/10.1038/s41598-017-14214-1( (2017).
Google Scholar
Chitraju, C. et al. Mice lacking triglyceride synthesis enzymes in adipose tissue are resistant to diet-induced obesity. eLife 12, RP88049. https://doi.org/10.7554/eLife.88049.3 (2023).
Google Scholar
Schoeler, M. & Caesar, R. Dietary lipids, gut microbiota and lipid metabolism. Rev. Endocr. Metab. Disord. 20, 461–472. https://doi.org/10.1007/s11154-019-09512-0 (2019).
Google Scholar
Brayton, C., Treuting, P. & Ward, J. Pathobiology of aging mice and GEM: background strains and experimental design. Vet. Pathol. 49, 85–105 (2012).
Google Scholar
Aldabbagh, E., Alhyali, H. & Ismaeel, H. The effects of ghee administration in comparison to sunflower seeds oil on liver tissue and some biochemical parameters in rats. Iraqi J. Vet. Sci. 36, 241–248 (2022).
Cho, Y. et al. Lipid remodeling of adipose tissue in metabolic health and disease. Exp. Mol. Med. 55, 1955–1973 (2023).
Google Scholar
Peña-Saldarriaga, L., Fernández-López, J. & Pérez-Alvarez, J. Quality of chicken fat by-products: Lipid profile and colour properties. Foods 9, 1046. https://doi.org/10.3390/foods9081046 (2020).
Google Scholar
Dal Bosco, A. et al. Indexing of fatty acids in poultry meat for its characterization in healthy human nutrition: a comprehensive application of the scientific literature and new proposals. Nutrients 14,3110. https://doi.org/10.3390/nu14153110 (2022).
German, J. et al. A reappraisal of the impact of dairy foods and milk fat on cardiovascular disease risk. Eur. J. Nutr. 48, 191–203. https://doi.org/10.1007/s00394-009-0002-5 (2009).
Google Scholar
Lawrence, G. Dietary fats and health: dietary recommendations in the context of scientific evidence. Adv. Nutr. 4, 294–302. https://doi.org/10.3945/an.113.003657 (2013).
Google Scholar
Duwaerts, C. & Maher, J. Macronutrients and the adipose-liver axis in obesity and fatty liver. Cell. Mol. Gastroenterol. Hepatol. 7, 749–761. https://doi.org/10.1016/j.jcmgh.2019.02.001 (2019).
Google Scholar
Jahan, M. et al. Comparative analysis of high-fat diets: effects of mutton, beef, and vegetable fats on body weight, biochemical profiles, and liver histology in mice. Heliyon 10, e39349. https://doi.org/10.1016/j.heliyon.2024.e39349 (2024).
Google Scholar
Palmquist, D. L. Great discoveries of milk for a healthy diet and a healthy life. R. Bras. Zootec. 39, 465–477. https://doi.org/10.1590/S1516-35982010001300051( (2010).
Zhao, B. et al. Plant and animal fat intake and overall and cardiovascular disease mortality. JAMA Intern. Med. 184, 1234–1245. https://doi.org/10.1001/jamainternmed.2024.3799 (2024).
Google Scholar
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